METHOD FOR ESTIMATING THE DEGREE OF CELL ANAPLASIA IN THE GLIOMA CULTURE Russian patent published in 2022 - IPC C12Q1/68 C12Q1/6844 C12Q1/686 C12Q1/6886 C12N5/09 

FIELD: biology.

SUBSTANCE: invention relates to the field of molecular biology and neurogenetics, in particular, to a method for estimating the degree of cell anaplasia in the glioma culture based on the analysis of gene expression. To implement the method, total RNA is first isolated from the samples; then reverse transcription is performed, followed by real-time RT-PCR amplification in order to determine the expression of the Sox2 and CDK6 genes. The ratio of expression of the Sox2/CDK6 genes is then calculated and the degree of cell anaplasia is estimated based on the value thereof. If said value is ≤0.11, the degree of cell anaplasia corresponds to grade II; if the value is greater than 0.11 but less than 0.7, the degree of cell anaplasia corresponds to grade III; and if the value is ≥0.7, grade IV cell anaplasia is apparent.

EFFECT: possibility of simplifying the process of diagnosing the degree of anaplasia of glioma cells and increasing the accuracy thereof.

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