FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Disclosed is a specific and highly sensitive test system for detecting the sheeppox virus genome. Oligonucleotide primers and a fluorescently labeled probe were developed for specific amplification and detection of a fragment of the ORF131-ORF132 sheeppox virus genome:
Forward TCGCATATGGAATTATCGGAAT
Reverse GATTTGACAAACACGTGTACAAA
probe FAM ACGAACATTTTTCCCTCTAAGCTACTTTAC BHQ1
The test system consists of a ready-made PCR mixture (including oligonucleotide primers and a fluorescently labeled probe), Taq-DNA polymerases, positive control and negative control and makes it possible to identify the sheeppox virus genome in the shortest possible time.
EFFECT: invention can be used to detect the DNA of all genetic variants of sheeppox viruses in samples of a wide range of pathological materials and biomaterials in order to make and clarify clinical and laboratory diagnoses in veterinary medicine.
1 cl, 1 dwg, 3 ex, 3 tbl
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