FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to molecular diagnostics. There are developed oligonucleotide primers and fluorescent-labeled probes for differentiation of genome of vaccine strain and field isolate of virus of infectious nodular dermatitis of cattle with additional detection of genome of capripox viruses by means of polymerase chain reaction in real time mode: field isolate of IND of cattle: f- 5'- TAGAAAATGGATGTACCACAAATACAG 3', r 5' -TTGTTACAACTCAAATCGTTAGGTG-3', probe 5' -ACCACCTAATGATAGTGTTTATGATTTACC-3; Capripox viruses: f - 5' ATGAAACCAATGGATGGGATA 3', r - 5' CGAAATGAAAAACGGTATATGGA 3', probe - 5' ATGAGCCATCCATTTTCCAA 3'4; vaccine strain of IND of cattle: f- 5'-TGTTTCCATTCTCCACTGCT-3', r - 5' -TACTTACTAAAAAATGGGCGCA-3', probe 5'-TCGCTGACATCGTTAGTCCACTC-3'. Disclosed is a method for differentiating the genome of a vaccine strain and a field isolate of the virus of infectious dermatoderma (nodular dermatitis) of cattle with additional detection of the genome of capripox viruses using real-time PCR at the same thermal profile using these primers and probes, and a PCR test system in real time for conducting thereof. A high-sensitivity PCR kit consisting of a ready PCR mixture, a Taq-polymerase enzyme, positive control and negative control, enabling specific detection and differentiation of the genome in the shortest possible time is developed.
EFFECT: disclosed are oligonucleotide primers and fluorescence-labeled probes, a method and a test system for differentiating the genome of the vaccine strain and the field isolate of the viral infectious dermatitis cattle with additional detection of the genome of capripox viruses using a real-time polymerase chain reaction.
3 cl, 3 dwg, 2 tbl, 3 ex
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