FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biotechnology. A device and a method for preparing donor fecal microbiota for transplantation under anaerobic conditions is proposed. The device contains a donor container, a mechanism for dosed supply of a solution and/or a cryoprotectant into the container, a mechanism for creating an anaerobic environment in the container, a homogenization mechanism, a mechanism for pumping out the obtained fecal microbiota from the container, and a control module. The method includes collecting the donor material into the donor container, weighing the container with the donor material, assessing the volume and dosed supply of the solution and/or cryoprotectant to the container, supplying carbon dioxide to the container, mixing the donor material and the solution and/or cryoprotectant in the container, filtering the resulting suspension, and dosed filling of containers for fecal microbiota.
EFFECT: inventions provide the possibility of obtaining microbiota samples of various composition and consistency from donor fecal material in sealed, anaerobic conditions from the very beginning of the process.
17 cl, 2 dwg
Title | Year | Author | Number |
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DEVICE FOR PREPARING DONOR FECAL MATERIAL FOR TRANSPLANTATION | 2021 |
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FECAL MICROBIOTA COMPOSITION FOR USE IN REDUCING TREATMENT-INDUCED INFLAMMATION | 2019 |
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RU2816462C2 |
ANALYTICAL METHOD FOR QUANTITATIVE ASSESSMENT OF VIABLE BACTERIA CONTAINED IN MICROBIOTA (MRT) RECOVERY COMPOSITIONS | 2017 |
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DEVICE FOR CRYOPRESERVATION AND PREPARATION OF THROMBOCYTES FOR TRANSFUSION | 2019 |
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Authors
Dates
2021-03-22—Published
2020-07-15—Filed