METHOD FOR PRODUCING COMPLEX FOR TUMOR THERAPEUTICS BASED ON ANTI-STOKES NANOPHOSPHORS AND PROTEIN MOLECULES Russian patent published in 2021 - IPC A61K51/02 A61P35/00 B82B3/00 B82Y5/00 B82Y40/00 A61K103/32 A61K103/36 

FIELD: medicine.

SUBSTANCE: invention relates to biomedicine, a method of producing a complex for tumour therapies based on anti-Stokes nanophosphors NaYF₄:Yb, Er/NaYF₄ and protein molecules, which can be used in diagnosing and treating tumours. Method for producing complex for tumour therapies based on anti-Stokes nanophosphors of core/shell configuration of composition NaYF₄:Yb, Er/NaYF₄ and protein molecules having histidine residues at the C-terminus of the molecule include modifying polyacrylic acid (CAA) with nitriloacetic acid (NTA) by adding EAC and NTA-L-lysine to the PAA to obtain the PAA-NTA product, removing oleic acid residues available on surface of nanophosphors, using nitrosyl tetrafluoroborate (NOBF₄), adding PAA-NTA to obtain PAA-NTA-coated nanoprophores. PAA-NTA-coated nanophosphors are then resuspended in deionised water, a nickel chloride solution is added to the suspension, the reaction mixture is treated with ultrasound and mixed. Nanophosphors are precipitated and washed with deionised water. Resuspending the nanophosphors in a recombinant protein solution having histidine residues at the C-terminus of the molecule to obtain complexes of anti-Stokes nanophosphors with a recombinant protein having histidine residues at the C-terminus of the molecule, due to non-covalent metal-affinity interaction between nickel ions and histidine residues.

EFFECT: high use of the invention to control the attachment of a protein molecule to the surface of nanophosphorus, which ensures preservation of functional properties of the protein and efficiency of the final complex.

1 cl, 2 dwg