FIELD: pharmaceuticals; medicine.
SUBSTANCE: subject 1 is a process for obtaining tumour antigen-specific T cells ex vivo, including: (a) depletion of CD14+ cells and CD25+ cells in a population of immune cells, including antigen-presenting cells (APC) and T cells, and the population of immune cells is taken from a biological sample of the subject - a person with cancer (b) incubating a CD14/CD25-depleted population of immune cells in the presence of: (i) an FMS-like tyrosine kinase 3 receptor ligand (FLT3L) and (ii) (A) a polypeptide containing at least two tumour antigen epitope sequences expressed by cancer cells a human subject, or (B) a polynucleotide encoding a polypeptide, thereby forming a population of stimulated T cells; and (c) growing a population of stimulated T cells. Subject 2 is a pharmaceutical composition for the treatment of cancer containing a grown population of CD14/CD25 depleted T cells.
EFFECT: formation of tumour antigen-specific CD8+ and CD4+ T cells, which leads to the generation of a population of antigen-specific T cells for therapeutic use for creating an immune response from naive CD8+ and CD4+ T cells.
18 cl, 43 dwg, 7 tbl, 28 ex
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