FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to a method for facilitating antibody-mediated antigen absorption by cells, including introduction of amino acid mutations into the said antibody. And the above antibody has the activity of binding human FcRn at pH 5.8 and pH 7. Also disclosed is a method for increasing the amount of antigens to which one antibody can bind, a method for increasing the ability of an antibody to eliminate antigen from plasma, a method for enhancing the elimination of antigen from plasma, a method for facilitating the extracellular release of an antigen-free form of an antibody taken up by a cell in an antigen-bound form, a method of reducing the total concentration of antigen or the concentration of free antigen in plasma, as well as a method for producing an antibody, providing for the introduction of the above mutations into the antibody. The invention also relates to a method for antibody screening that provides for selecting an antibody that has a human FcRn binding activity at pH 5.8 and pH 7.0 and a higher human FcRn binding activity at pH 7.0.
EFFECT: invention makes it possible to effectively obtain an antibody with improved pharmacokinetics.
11 cl, 35 dwg, 15 tbl, 17 ex
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