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2 747 820

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(51)

IPC

C12Q1/6816(2018-01-01)

C12N9/22(2006-01-01)

C12N15/113(2010-01-01)

(21) (22)

Application

2020139162, 2020-11-30

(24)

Start date

2020-11-30

(22)

Actual filing date

2020-11-30

(45)

Published

2021-05-14

(72)

Inventor

Tiumentsev Aleksandr IgorevichTiumentseva Marina AlekseevnaAkimkin Vasilii Gennadevich

(73)

Holder

Federalnoe Biudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatelskii Institut Epidemiologii» Federalnoi Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelei I Blagopoluchiia Cheloveka Tsnii Epidemiologii Rospotrebnadzora)

CRISPR-CAS SYSTEM FOR DETECTION OF JOHN CUNNINGHAM VIRUS (JCPYV) DNA AT ULTRA-LOW CONCENTRATIONS Russian patent published in 2021 - IPC C12Q1/6816 C12N9/22 C12N15/113

Abstract RU 2747820 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, namely to guide RNAs, ribonucleoprotein complexes of the CRISPR / CAS system containing guide RNAs, and kits containing ribonucleoprotein complexes of the CRISPR / CAS system and specific oligonucleotides for preliminary amplification of highly conserved regions of the John Cunningham virus genome (JCP). EFFECT: invention makes it possible to efficiently detect DNA of John Cunningham virus (JCPyV) after carrying out specific amplification of fragments of this DNA.

EFFECT: detection of single DNA copies of John Cunningham virus (JCPyV).

7 cl, 6 dwg, 4 tbl, 5 ex

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RU 2 747 820 C1

Authors

Tiumentsev Aleksandr Igorevich

Tiumentseva Marina Alekseevna

Akimkin Vasilii Gennadevich

Dates

2021-05-14—Published

2020-11-30—Filed