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2 802 783

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(51)

IPC

C12N15/113(2010-01-01)

G01N33/50(2006-01-01)

C12Q1/68(2006-01-01)

(21) (22)

Application

2022134900, 2022-12-28

(24)

Start date

2022-12-28

(22)

Actual filing date

2022-12-28

(45)

Published

2023-09-01

(72)

Inventor

Tiumentsev Aleksandr IgorevichTiumentseva Marina AlekseevnaPrelovskaia Anna NikolaevnaAkimkin Vasilii Gennadevich

(73)

Holder

Federalnoe Biudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatelskii Institut Epidemiologii» Federalnoi Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelei I Blagopoluchiia Cheloveka Tsnii Epidemiologii Rospotrebnadzora)

CRISPR-CAS12 SYSTEM FOR DETECTING HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) RNA IN ULTRA-LOW CONCENTRATIONS IN ULTRA-LOW CONCENTRATIONS Russian patent published in 2023 - IPC C12N15/113 G01N33/50 C12Q1/68

Abstract RU 2802783 C1

FIELD: genetic engineering; biotechnology.

SUBSTANCE: invention relates to the field of genetic engineering and biotechnology, namely to guide RNAs, ribonucleoprotein complexes of the CRISPR-Cas system containing guide RNAs, and kits containing ribonucleoprotein complexes of the CRISPR-Cas system and specific oligonucleotides for pre-amplification of highly conserved sections of the human immunodeficiency virus (HIV-1) genome. The invention makes it possible to effectively detect the RNA of the human immunodeficiency virus (HIV-1) after specific amplification of fragments of the genome of this virus.

EFFECT: invention provides detection of single copies of the human immunodeficiency virus (HIV-1) RNA. 7.

7 cl, 8 dwg, 4 tbl, 5 ex

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RU 2 802 783 C1

Authors

Tiumentsev Aleksandr Igorevich

Tiumentseva Marina Alekseevna

Prelovskaia Anna Nikolaevna

Akimkin Vasilii Gennadevich

Dates

2023-09-01—Published

2022-12-28—Filed