FIELD: genetic engineering; biotechnology.
SUBSTANCE: invention relates to the number of means — guide RNA, ribonucleoprotein complexes of the CRISPR-Cas system containing guide RNA, diagnostic methods and kits containing ribonucleoprotein complexes of the CRISPR-Cas system and specific oligonucleotides for preliminary amplification of highly conservative regions human immunodeficiency virus (HIV-1) genome.
The invention enables effective detection of human immunodeficiency virus (HIV-1) genome RNA after specific amplification of the virus genome fragments. Guide RNAs are represented by the sequences of the following SEQ ID NO: 1-5, specific oligonucleotides for pre-amplification are represented by the sequences of the following SEQ ID NO: 6-7).
EFFECT: invention provides detection of single copies of RNA of human immunodeficiency virus (HIV-1) genome.
5 cl, 8 dwg, 4 tbl, 2 ex
