METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD Russian patent published in 2023 - IPC C12N15/113 C12Q1/68 G01N33/50 

Abstract RU 2802079 C1

FIELD: genetic engineering; biotechnology.

SUBSTANCE: invention relates to the number of means — guide RNA, ribonucleoprotein complexes of the CRISPR-Cas system containing guide RNA, diagnostic methods and kits containing ribonucleoprotein complexes of the CRISPR-Cas system and specific oligonucleotides for preliminary amplification of highly conservative regions human immunodeficiency virus (HIV-1) genome.

The invention enables effective detection of human immunodeficiency virus (HIV-1) genome RNA after specific amplification of the virus genome fragments. Guide RNAs are represented by the sequences of the following SEQ ID NO: 1-5, specific oligonucleotides for pre-amplification are represented by the sequences of the following SEQ ID NO: 6-7).

EFFECT: invention provides detection of single copies of RNA of human immunodeficiency virus (HIV-1) genome.

5 cl, 8 dwg, 4 tbl, 2 ex

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RU 2 802 079 C1

Authors

Tiumentsev Aleksandr Igorevich

Tiumentseva Marina Alekseevna

Prelovskaia Anna Nikolaevna

Vlasenko Natalia Viktorovna

Kuzin Stanislav Nikolaevich

Akimkin Vasilii Gennadevich

Dates

2023-08-22Published

2022-12-28Filed