FIELD: genetic engineering; biotechnology.
SUBSTANCE: guide RNA molecule of the CRISPR-Cas system, a ribonucleoprotein complex of the CRISPR-Cas system for detecting hepatitis C virus RNA, and kits for detecting hepatitis C virus RNA are proposed. A guide RNA molecule of the CRISPR-Cas system can bind to targeted highly conserved genome sites of hepatitis C virus and has a consequence selected from SEQ ID NO: a sequence selected from SEQ ID NO: 1-5. The ribonucleoprotein complex of the CRISPR-Cas system is formed from the RNA-guided DNA endonuclease LbCpf1 from Lachnospiraceae and at least one guide RNA. The kits contain a CRISPR-Cas guide RNA molecule, guided DNA endonuclease LbCpf1 from Lachnospiraceae, instructions for use, and additionally one or more specific oligonucleotides selected from SEQ ID NO: 6-11.
EFFECT: inventions provide ultrasensitive detection of hepatitis C virus RNA of genotypes 1b and 3a up to single (2,3) copies of RNA in one reaction.
7 cl, 11 dwg, 6 tbl, 5 ex
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|---|---|---|---|
| METHOD OF PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-CAS AND A PREPARATION FOR DETECTING HEPATITIS C VIRUS RNA OF GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2799430C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
|
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| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS AT ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
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| METHOD FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
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| METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
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RU2820307C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA, AT ULTRA-LOW CONCENTRATIONS | 2021 |
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RU2791879C1 |
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RU2802783C1 |
