FIELD: genetic engineering; biotechnology.
SUBSTANCE: group of inventions relates to obtaining ribonucleoprotein complexes of the CRISPR-Cas system. A method of obtaining a preparation of the CRISPR-Cas ribonucleoprotein complex and a preparation of the CRISPR-Cas ribonucleoprotein complex are proposed. The following is performed: synthesis of guide RNA with SEQ ID NO: 1-5. The Cas protein of the CRISPR-Cas LbCpf1 family from Lachnospiraceae is complexed with at least one resulting guide RNA.
EFFECT: inventions provide detection of single copies of hepatitis C virus RNA of genotypes 1b and 3a.
6 cl, 11 dwg, 6 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CRISPR-CAS12 SYSTEM FOR DETECTING HEPATITIS C VIRUS RNA GENOTYPES 1B AND 3A AT ULTRA-LOW CONCENTRATIONS | 2022 |
|
RU2800421C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
|
RU2800420C1 |
| METHOD FOR PRODUCING AN AGENT OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas12 AND AGENT FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2792891C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTION OF HEPATITIS B VIRUS DNA AT ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2782700C1 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS AT ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2782951C1 |
| METHOD FOR OBTAINING A PREPARATION OF THE CRISPR/Cas 12 RIBONUCLEOPROTEIN COMPLEX AND PREPARATION FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2764015C1 |
| METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2820307C1 |
| METHOD FOR DETECTING SARS-CoV-2 VIRUS RNA IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2764021C1 |
| METHOD FOR PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND A PREPARATION FOR DETECTING THE exoU GENE ENCODING AN EXOTOXIN OF THE THIRD TYPE SECRETION SYSTEM, PSEUDOMONAS AERUGINOSA | 2021 |
|
RU2782739C1 |
| METHOD FOR OBTAINING A PREPARATION OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND A PREPARATION FOR DETECTING THE mecA ANTIBIOTIC RESISTANCE GENE OF STAPHYLOCOCCUS AUREUS IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2782315C1 |
