FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a minimally invasive method for differential early diagnosis of hepatocellular carcinoma and fatty hepatosis. Said method involves extraction of extracellular DNA from blood plasma, determination of copy number of genes GOT1, CYC1, ALPL relative to reference gene GAPDH by real-time PCR in the presence of EVA-Green dye and highly specific primers with sequences SEQ ID NO: 1–8, calculation of relative gene copy number (rC) and comparison of obtained rC values with prognostic copy number interval.
EFFECT: present invention provides a simple, inexpensive and accurate method with highly specific primer sequences for minimally invasive differential early diagnosis of hepatocellular carcinoma and fatty hepatosis.
1 cl, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MINIMALLY INVASIVE METHOD FOR DIAGNOSIS OF HIGH-GRADE SEROUS OVARIAN ADENOCARCINOMA BASED ON COPY INDEX OF SULT1E1, CYP1B1, AND ESR1 GENES | 2021 |
|
RU2750472C1 |
| MINIMALLY INVASIVE METHOD FOR DETECTING SENSITIVITY OF PROSTATE TUMOURS TO RADIATION THERAPY BASED ON CHANGE IN BRCA2 AND RAD50 GENE COPYING | 2019 |
|
RU2728428C1 |
| MINIMALLY INVASIVE METHOD FOR DETECTING SENSITIVITY OF RECTAL TUMOUR TO RADIATION THERAPY BASED ON CHANGE IN ABUNDANCE OF N2AX AND RBBP8 GENES | 2019 |
|
RU2740576C1 |
| METHOD OF DETECTING DNAJB1-PRKACA CHIMERIC TRANSCRIPT IN CLINICAL TISSUE SAMPLES FROM PATIENTS WITH FIBROLAMELLAR LIVER CARCINOMA USING REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2807306C1 |
| MINIMALLY INVASIVE METHOD FOR DIAGNOSING METASTATIC LESIONS OF REGIONAL LYMPH NODES IN PATIENTS WITH CERVICAL CANCER BASED ON THE COPY NUMBER OF THE CCND1 AND PPARGC1A GENES | 2021 |
|
RU2766774C1 |
| MINIMALLY INVASIVE METHOD FOR DIAGNOSTICS OF LUNG CANCER ON BASIS OF CHANGING COPY NUMBER OF MTDNA HV2 LOCUS | 2018 |
|
RU2678227C1 |
| TEST SYSTEM FOR DETERMINING IDH 1/2 GENE STATUS IN GLIAL TUMOR TISSUES | 2023 |
|
RU2823028C1 |
| METHOD OF PROGNOSTIC ASSESSMENT OF DEVELOPMENT OF HEPATOCELLULAR CARCINOMA BASED ON DETERMINATION OF POLYMORPHISM OF HUMAN IFNAR-1 GENE AND A SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS | 2022 |
|
RU2811763C1 |
| OLIGONUCLEOTIDES FOR DIAGNOSING EPILEPSY BY QUANTITATIVE PCR | 2023 |
|
RU2815113C1 |
| METHOD FOR DIAGNOSIS OF DISEASE ACCOMPANIED BY INCREASED CELL DEATH, AND SET FOR ITS IMPLEMENTATION | 2016 |
|
RU2630648C2 |
