FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a minimally invasive method for differential early diagnosis of hepatocellular carcinoma and fatty hepatosis. Said method involves extraction of extracellular DNA from blood plasma, determination of copy number of genes GOT1, CYC1, ALPL relative to reference gene GAPDH by real-time PCR in the presence of EVA-Green dye and highly specific primers with sequences SEQ ID NO: 1–8, calculation of relative gene copy number (rC) and comparison of obtained rC values with prognostic copy number interval.
EFFECT: present invention provides a simple, inexpensive and accurate method with highly specific primer sequences for minimally invasive differential early diagnosis of hepatocellular carcinoma and fatty hepatosis.
1 cl, 1 tbl, 2 ex
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