METHOD FOR DIFFERENTIAL DIAGNOSIS OF SQUAMOUS CELL CARCINOMA OF TONGUE AND BENIGN NEOPLASM OF TONGUE Russian patent published in 2025 - IPC G01N33/58 C12Q1/6806 C12Q1/686 C12Q1/6876 C12Q1/6886 

FIELD: medical science.

SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for differential diagnosis of squamous cell carcinoma of tongue and benign growth of tongue. Cell-free DNA (cfDNA) is recovered from blood plasma. Copy number of MMP1 and MAL genes with respect to the reference gene GAPDH is determined by real-time PCR in the presence of high-specific primers on a matrix of the recovered cfDNA. Relative gene copy number (rC) is calculated, and the derived rC values are compared to a prognostic copy number interval. At values rC_MMP1>5.52*10^-3 and rC_MAL<4.01*10^-4, squamous cell tongue cancer is diagnosed. At values rC_MMP1<5.05*10^-3 and rC_MAL>4.21*10^-4, benign growth of tongue is diagnosed.

EFFECT: method provides simple, accurate and minimally invasive diagnosis of squamous cell carcinoma of tongue ensured by determining the copy number of MMP1 and MAL genes in blood.

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