METHOD FOR OBTAINING AN ERYTHROCYTE DIAGNOSTICUM FOR THE INDIRECT HEMAGGLUTINATION REACTION (IHAR) WITH CLOSTRIDIOSIS OF ANIMALS Russian patent published in 2021 - IPC G01N33/531 A61K39/08 

FIELD: biotechnology, veterinary medicine, immunology.

SUBSTANCE: a method for obtaining an erythrocyte diagnosticum for the reaction of indirect hemagglutination (IHAR) in animal clostridiosis is proposed, including sensitization of sheep erythrocytes containing formalin and tannin with a mixture of anatoxins of five Cl strains. perfringens No. 28 (type A), LD-1 (type B), No. 392 (type C), No. 213 (type D), No. 342 (type E) in equal proportions obtained from the culture medium by precipitation of formalin-inactivated toxins at a temperature of 37°С (anatoxins) with ammonium sulfate, and somatic antigens obtained by exposure to these Cl. perfringens strains with ultrasound on a disintegrator at a frequency of 20 kHz±500 Hz for 10 min at a temperature of 4°С.

EFFECT: provides an increase in the specificity and sensitivity of methods for laboratory diagnostics of anaerobic enterotoxemia (clostridiosis) of unvaccinated livestock of farm animals and control of the intensity of post-vaccination immunity in vaccinated animals.

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