METHOD FOR OBTAINING COORDINATION COMPLEX OF THE Gd3 + ION WITH SYNTHETIC RECOMBINANT PROTEIN IN ORDER TO OBTAIN CONTRASTING AGENT FOR MAGNETIC RESONANCE IMAGING Russian patent published in 2021 - IPC C12N15/00 C07K1/18 A61K33/244 C12N15/70 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. A method for obtaining a strong complex of the Gd³⁺ ion with an artificial recombinant W4 protein is described. The method differs in that the purified W4 protein is applied to a column with an ion-exchange sorbent in a ratio of 1:0.5 to 1:2 mg per ml of the sorbent in a HEPES buffer with a concentration of 10-20 mM and a pH of 7.0-7.4, and gadolinium nitrate at a concentration of 1 mM is applied in a buffer to balance the column in a ratio of 0.2 ml per 1 ml of the sorbent, while the ratio in the complex is 1:12.193±0.1102 mol Gd³⁺ per mol of protein.

EFFECT: invention expands the arsenal of methods for obtaining Gd³ + ion complexes with recombinant proteins suitable for contrasting tumors using paramagnetic resonance spectroscopy, as well as for radiotherapy of tumors on the principle of thermal ablation.

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