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2 756 472

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IPC

C12Q1/68(2006-01-01)

C12N1/00(2006-01-01)

(21) (22)

Application

2020129954, 2020-09-10

(24)

Start date

2020-09-10

(22)

Actual filing date

2020-09-10

(45)

Published

2021-09-30

(72)

Inventor

Doronin Maksim IgorevichMikhalishin Dmitrij ValerevichStarikov Vyacheslav AlekseevichBorisov Aleksej ValerevichMudrak Natalya Stanislavovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD FOR MEDIATED DETERMINATION OF THE INFECTIOUS TITER OF THE RABIES VIRUS IN NON-INACTIVATED RAW MATERIALS FOR RABIES VACCINES IN TRANSCRIPTIONAL AMPLIFICATION AND DETECTION OF REACTION PRODUCTS USING THE BEACON TECHNOLOGY Russian patent published in 2021 - IPC C12Q1/68 C12N1/00

Abstract RU 2756472 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, namely to a method for mediated determination of the infectious titer of the rabies virus in non-inactivated raw materials for rabies vaccines in transcriptional amplification and detection of reaction products using the beacon technology and the developed logarithmic function of the dependence of the value of the threshold cycle of transcriptional amplification of RNA and the infectious titer of the rabies virus. The value of the infectivity titer of the rabies virus is determined while conducting denaturation and the reaction of transcriptional amplification of viral RNA using original specific oligonucleotides by the value of the threshold cycle of amplification of RNA.

EFFECT: developed method for determining the infectious titer of the rabies virus using the beacon technology.

7 cl, 4 dwg, 9 tbl, 5 ex

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RU 2 756 472 C1

Authors

Doronin Maksim Igorevich

Mikhalishin Dmitrij Valerevich

Starikov Vyacheslav Alekseevich

Borisov Aleksej Valerevich

Mudrak Natalya Stanislavovna

Dates

2021-09-30—Published

2020-09-10—Filed