METHOD FOR THE INDIRECT DETERMINATION OF THE CONCENTRATION OF THE IMMUNOGENIC RNP COMPLEX OF RABIES VIRUS IN RAW MATERIALS FOR RABIES VACCINES BY AMPLIFICATION AND HYBRIDIZATION-FLUORESCENT DETECTION OF DNA AMPLICONS Russian patent published in 2021 - IPC C12Q1/68 C12Q1/00 

Abstract RU 2760436 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology and the production of rabies vaccines, namely, to a method for indirectly determining the concentration of the immunogenic RNP complex of rabies virus in raw materials for rabies vaccines by amplification and hybridization-fluorescent detection of DNA amplicons using original specific oligonucleotides and the developed linear function of the dependence of the threshold amplification cycle and the concentration of RNP complex.

EFFECT: proposed invention makes it possible to quickly and with a high degree of reliability determine the concentrations of the RNP complex of the rabies virus in raw materials for anti-rabies vaccines.

9 cl, 6 dwg, 10 tbl, 6 ex.

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RU 2 760 436 C1

Authors

Doronin Maksim Igorevich

Mikhalishin Dmitrij Valerevich

Starikov Vyacheslav Alekseevich

Borisov Aleksej Valerevich

Metlin Artem Evgenevich

Elkina Yuliya Sergeevna

Dates

2021-11-25Published

2020-08-03Filed