METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "PB-97" FROM FIELD RABIES VIRUS ISOLATES BY DATA OF MAXIMUM GRAPH OF DERIVATIVE OF SIGMOID FUNCTION DURING AMPLIFICATION OF SECTION OF N-GENE OF VIRAL CDNA USING SYTO 16 DYE Russian patent published in 2024 - IPC G01N33/569 C12N15/00 

Abstract RU 2823779 C1

FIELD: veterinary virology.

SUBSTANCE: described is a method of differentiating the vaccine strain "PB-97" from field isolates of rabies virus according to the maximum of the graph of the derivative of the sigmoid function when amplifying a portion of the N-gene of the viral cDNA using SYTO 16 dye. Method is characterized by analytical specificity equal to 100%, diagnostic sensitivity in 95% confidence interval equal to 99.19–100.00%, diagnostic specificity—98.44–100.0%, overall accuracy—99.47–100.00%.

EFFECT: main advantage of the present invention is the possibility of differentiating the genome of the vaccine strain “PB-97” from field isolates of the rabies virus in a short period of time of 2.5 hours using the developed method.

2 cl, 3 dwg, 5 tbl, 3 ex

Similar patents RU2823779C1

Title Year Author Number
METHOD FOR DIFFERENTIATION OF GENOME OF ARRIAH VACCINE STRAIN FROM FIELD ISOLATES OF RABIES VIRUS BY POLYMERASE CHAIN REACTION IN REAL TIME WITH ANALYSIS OF PEAKS OF MELTING TEMPERATURES OF AMPLICONS AND USE OF ASYMMETRIC DYE SYBR GREEN 2023
  • Doronin Maksim Igorevich
  • Malygin Maksim Pavlovich
  • Mikhalishin Dmitrij Valerevich
  • Chvala Ilya Aleksandrovich
  • Borisov Aleksej Valerevich
  • Prokhvatilova Larisa Borisovna
  • Ruchnova Olga Ivanovna
RU2822037C1
METHOD OF DIFFERENTIATING VACCINE STRAIN "RICH" FROM OTHER STRAINS AND FIELD ISOLATES OF CAUSATIVE AGENT OF CORONAVIRUS INFECTION IN DOGS BY ANALYZING MAXIMUM EXTREMA OF AMPLICON MELTING TEMPERATURE CURVES USING SUPERNOVA v428 VIOLET LASER DYE 2023
  • Doronin Maksim Igorevich
  • Galkina Tatyana Sergeevna
  • Mikhalishin Dmitrij Valerevich
  • Chvala Ilya Aleksandrovich
  • Isakova Darya Georgievna
  • Borisov Aleksej Valerevich
  • Shevchenko Maksim Aleksandrovich
  • Prokhvatilova Larisa Borisovna
RU2826458C1
METHOD FOR MEDIATED DETERMINATION OF THE INFECTIOUS TITER OF THE RABIES VIRUS IN NON-INACTIVATED RAW MATERIALS FOR RABIES VACCINES IN TRANSCRIPTIONAL AMPLIFICATION AND DETECTION OF REACTION PRODUCTS USING THE BEACON TECHNOLOGY 2020
  • Doronin Maksim Igorevich
  • Mikhalishin Dmitrij Valerevich
  • Starikov Vyacheslav Alekseevich
  • Borisov Aleksej Valerevich
  • Mudrak Natalya Stanislavovna
RU2756472C1
METHOD OF DIFFERENTIATING ARRIAH-G333 GENOME OF GENETICALLY MODIFIED STRAIN FROM PB-97 VACCINE STRAIN OF RABIES VIRUS USING REAL-TIME POLYMERASE CHAIN REACTION WITH HIGH-RESOLUTION PEAK ANALYSIS 2023
  • Chupin Sergej Aleksandrovich
  • Doronin Maksim Igorevich
  • Mazlum Ali
  • Sprygin Aleksandr Vladimirovich
RU2812859C1
METHOD FOR THE INDIRECT DETERMINATION OF THE CONCENTRATION OF THE IMMUNOGENIC RNP COMPLEX OF RABIES VIRUS IN RAW MATERIALS FOR RABIES VACCINES BY AMPLIFICATION AND HYBRIDIZATION-FLUORESCENT DETECTION OF DNA AMPLICONS 2020
  • Doronin Maksim Igorevich
  • Mikhalishin Dmitrij Valerevich
  • Starikov Vyacheslav Alekseevich
  • Borisov Aleksej Valerevich
  • Metlin Artem Evgenevich
  • Elkina Yuliya Sergeevna
RU2760436C1
METHOD OF INDIRECT DETERMINING TITER OF INFECTIOUS ACTIVITY OF RABIES VIRUS IN RAW MATERIALS FOR PRODUCTION OF ATTENUATED RABIES VACCINE USING TECHNOLOGY OF ALGEBRAIC ANALYSIS OF SECOND-ORDER DIFFERENTIAL OF MAXIMUM POINT d CPmax OF PCR LOGISTIC CURVE 2023
  • Doronin Maksim Igorevich
  • Malygin Maksim Pavlovich
  • Mikhalishin Dmitrij Valerevich
  • Chvala Ilya Aleksandrovich
  • Borisov Aleksej Valerevich
  • Razgulyaeva Evgeniya Aleksandrovna
  • Gochmuradov Ykhlas Muradovich
RU2811995C1
METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605 2024
  • Doronin Maksim Igorevich
  • Galkina Tatiana Sergeevna
  • Mikhalishin Dmitrii Valerevich
  • Guseva Marina Nikolaevna
  • Okovytaia Tatiana Vladimirovna
  • Elkina Iuliia Sergeevna
  • Budina Olesia Olegovna
  • Gochmuradov Ykhlas Muradovich
  • Koroleva Kseniia Valerevna
RU2824661C1
METHOD FOR INDIRECT DETERMINATION OF THE NUMBER OF INFECTIOUS DOSES OF RABIES VIRUS STRAIN PB -97 IN RAW MATERIALS FOR ATTENUATED RABIES VACCINE BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN REACTION IN REAL TIME 2020
  • Doronin Maksim Igorevich
  • Mikhalishin Dmitrij Valerevich
  • Borisov Aleksej Valerevich
  • Starikov Vyacheslav Alekseevich
RU2761535C1
ANTIRABIC INACTIVATED EMULSION CULTURE VACCINE FOR PROPHYLACTIC IMMUNIZATION OF DOMESTIC CARNIVORES AND FARM ANIMALS 2019
  • Lozovoj Dmitrij Anatolevich
  • Mikhalishin Dmitrij Valerevich
  • Borisov Aleksej Valerevich
  • Doronin Maksim Igorevich
  • Balashov Andrej Nikolaevich
  • Starikov Vyacheslav Alekseevich
RU2722868C1
METHOD FOR DIFFERENTIATING THE GENOME OF THE NISHI VACCINE STRAIN FROM FIELD ISOLATES OF SHEEP POX VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION WITH HIGH-RESOLUTION PEAK ANALYSIS 2022
  • Sprygin Aleksandr Vladimirovich
  • Krotova Alena Olegovna
  • Mazlum Ali
  • Shalina Kseniya Aleksandrovna
  • Prutnikov Pavel Vladimirovich
  • Nesterov Aleksandr Aleksandrovich
  • Shumilova Irina Nikolaevna
  • Doronin Maksim Igorevich
  • Byadovskaya Olga Petrovna
RU2786213C1

RU 2 823 779 C1

Authors

Doronin Maksim Igorevich

Malygin Maksim Pavlovich

Mikhalishin Dmitrij Valerevich

Chvala Ilya Aleksandrovich

Shishkova Anzhela Alekseevna

Borisov Aleksej Valerevich

Shishkov Aleksandr Valerevich

Dates

2024-07-30Published

2023-08-29Filed