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2 823 779

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IPC

G01N33/569(2006-01-01)

C12N15/00(2006-01-01)

(21) (22)

Application

2023122523, 2023-08-29

(24)

Start date

2023-08-29

(22)

Actual filing date

2023-08-29

(45)

Published

2024-07-30

(72)

Inventor

Doronin Maksim IgorevichMalygin Maksim PavlovichMikhalishin Dmitrij ValerevichChvala Ilya AleksandrovichShishkova Anzhela AlekseevnaBorisov Aleksej ValerevichShishkov Aleksandr Valerevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "PB-97" FROM FIELD RABIES VIRUS ISOLATES BY DATA OF MAXIMUM GRAPH OF DERIVATIVE OF SIGMOID FUNCTION DURING AMPLIFICATION OF SECTION OF N-GENE OF VIRAL CDNA USING SYTO 16 DYE Russian patent published in 2024 - IPC G01N33/569 C12N15/00

Abstract RU 2823779 C1

FIELD: veterinary virology.

SUBSTANCE: described is a method of differentiating the vaccine strain "PB-97" from field isolates of rabies virus according to the maximum of the graph of the derivative of the sigmoid function when amplifying a portion of the N-gene of the viral cDNA using SYTO 16 dye. Method is characterized by analytical specificity equal to 100%, diagnostic sensitivity in 95% confidence interval equal to 99.19–100.00%, diagnostic specificity—98.44–100.0%, overall accuracy—99.47–100.00%.

EFFECT: main advantage of the present invention is the possibility of differentiating the genome of the vaccine strain “PB-97” from field isolates of the rabies virus in a short period of time of 2.5 hours using the developed method.

2 cl, 3 dwg, 5 tbl, 3 ex

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RU 2 823 779 C1

Authors

Doronin Maksim Igorevich

Malygin Maksim Pavlovich

Mikhalishin Dmitrij Valerevich

Chvala Ilya Aleksandrovich

Shishkova Anzhela Alekseevna

Borisov Aleksej Valerevich

Shishkov Aleksandr Valerevich

Dates

2024-07-30—Published

2023-08-29—Filed