METHOD FOR DETECTION AND CLASSIFICATION OF PROTEIN MOLECULES AND THEIR INTERACTIONS Russian patent published in 2021 - IPC G01N21/25 

FIELD: analytical chemistry.

SUBSTANCE: invention relates to analytical chemistry, in particular to methods for the detection and classification of protein molecules and products of their interactions in solutions based on optical sensor devices without the use of analytical labels. A method for detecting interactions of protein molecules with a specific binding site includes preparing model samples, which are at least one solution of a detected protein, a solution of a specific binding site and a mixture of a protein solution with solutions of specific binding sites with different concentrations, introducing solutions and mixtures into the internal cavity of a microstructured optical fibers, detection of transmission spectra of solutions and mixtures, which are used to consider the presence of interactions, in this case, the detection of the transmission spectra is carried out in the spectral range from 250 nm to 3000 nm, the data of the transmission spectra of the model samples are used to construct a mathematical model of the interaction of the detected proteins, for this, the data of the spectra are presented in the form of a matrix, the rows of which correspond to the sample numbers, and the columns - to the intensities of the spectral signal, the matrix is decomposed by the method for principal components, graphs of scores and loads are obtained, on which groups of points characterizing the presence or absence of interaction are distinguished, a test solution and mixtures of a test solution with a specific binding site with different concentrations are prepared, the test solution and mixtures are introduced into the internal cavities of the microstructured optical fiber and detect the transmission spectra, which are compared with the spectra of the model samples, and when the spectral data of the tested solutions fall into the corresponding group of points of the model samples, the presence or absence of interaction is considered.

EFFECT: reduction of the analysis time, increase in the accuracy and reliability of the detection and classification of protein molecules and products of their interactions in solutions.

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