LIPOSOMAL FORM OF ABIES SIBIRICA RESIN FOR NON-INVASIVE DELIVERY OF BIOLOGICALLY ACTIVE SUBSTANCE OF ABIES SIBIRICA OLEORESIN TO BRAIN, WHICH HAS ANTIOXIDANT, CYTOTOXIC ACTIVITY AGAINST CERVICAL CANCER CELLS, AND METHOD FOR ITS PREPARATION Russian patent published in 2021 - IPC A61K9/127 A61K36/15 A61K47/24 A61K47/28 A61K47/60 A61P35/00 A61P17/18 B82B3/00 

FIELD: molecular medicine; pharmacology.

SUBSTANCE: invention relates to the field of molecular medicine and pharmacology, namely to a liposomal form of Abies sibirica resin. A method for obtaining the liposomal form of Abies sibirica resin, which is phospholipid membrane with the inclusion of natural Abies sibirica resin (oleoresin) in amount of up to 1% by wt., is proposed including following stages: simple mixing of 0.55 g of phospholipid, 0.03 g of cholesterol, 0.04 g of a pegylating agent - up to 6% by wt. and active substance - Abies sibirica resin - up to 1% by wt. in a suitable solvent, evaporation of the solvent at a temperature above a phospholipid phase transition to obtain homogeneous lipid film, its rehydration in a medium selected from ultra-purified water or a physiological 0.9% aqueous solution of sodium chloride - up to 100% by wt., while mixing at a constant temperature above the phospholipid phase transition and subsequent dispersion of particles to a size of about 100 nm. The liposomal form of Abies sibirica resin obtained by the above-described method has an activity selected from: a function of directed delivery of biologically active substance of Abies sibirica oleoresin to the brain, antioxidant activity in vitro, cytotoxic activity in vitro against human tumor cells M-Hela. The liposomal form of Abies sibirica resin is used to ensure the activity selected from: penetration into the central nervous system (CNS), penetration into the CNS with intranasal administration, antioxidant activity in vitro, cytotoxic activity in vitro against human tumor cells M-Hela.

EFFECT: above-described liposomal form of Abies sibirica resin has an increased bioavailability of Abies sibirica resin, which is water-soluble, is capable of non-invasively penetrating the CNS and at the same time has pharmacological activity, simultaneously exhibiting in vitro antioxidant properties and cytotoxic activity against cervical cancer cells M-Hela.

8 cl, 15 dwg, 3 tbl, 7 ex