METHOD OF CLONAL MICROPROPAGATION OF PHLOX PANICULATA Russian patent published in 2021 - IPC A01H4/00 

Abstract RU 2743966 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. The invention constitutes a method that involves planting microplants on a nutrient medium with mineral salts for the proliferation stage in accordance with the method of the Murashige and Skoog (MS). Kinetin (0.1 mg/l) and IBA (0.1 mg/l) are added to the nutrient medium. The microplants are incubated for 50-60 days in a light room at an illumination intensity of 2500 lx, a 16-hour photoperiod and a temperature of 20-22C. Then micro-cutting is carried out in which the lower rooted part of the regenerated plants is sent for adaptation to non-sterile conditions, and the upper part is divided into single-node segments and planted on a fresh nutrient medium for further replication.

EFFECT: invention will allow speeding up production process by 65-70 days by combining the stages of proliferation and rhizogenesis.

1 cl, 3 dwg, 3 tbl

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RU 2 743 966 C1

Authors

Mazaeva Anna Sergeevna

Akimova Svetlana Vladimirovna

Kovaleva Irina Sergeevna

Matsneva Anna Evgenevna

Khanbabaeva Olga Evgenevna

Dates

2021-03-01Published

2020-06-09Filed