MEDIA AND METHODS FOR FERMENTATION FOR PRODUCING POLYSACCHARIDES IN BACTERIAL CELL CULTURE Russian patent published in 2021 - IPC C12N1/20 C12P19/04 C12R1/46 

FIELD: microbiology.

SUBSTANCE: group of inventions relates to a polysaccharide-producing bacterial medium for cultivating Streptococcus pneumoniae or Streptococcus agalactiae and its use. A polysaccharide-producing bacterial medium for cultivating Streptococcus pneumoniae or Streptococcus agalactiae is proposed, containing the total concentration of amino acid of at least 60 mM per 1 l of a cell culture medium, the total concentration of glycine from 1.5 mM to 60 mM per 1 l of the cell culture medium, the total concentration of potassium chloride from 0.3 to 24 g/l, and a glucose source. The specified total concentration of amino acid contains amino acid selected from arginine, asparagine, cysteine, glutamine, glycine, serine and tyrosine. The specified medium is used in a method for cultivating bacteria producing polysaccharides selected from Streptococcus pneumoniae and Streptococcus agalactiae.

EFFECT: group of inventions provides for an improvement of growth and production of capsular polysaccharide by Streptococcus pneumoniae or Streptococcus agalactiae, when cultivating.

34 cl, 29 tbl, 17 ex