FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biotechnology, in particular to immunoglobulin conjugates. The invention allows one, during an enzymatic reaction catalyzed with microbial transglutaminase, to obtain a conjugate of lysine containing a modified or mutant residue in a constant region of immunoglobulin with a functional means containing acyl-donor substrate with glutamine residue.
EFFECT: use of the site-specific enzymatic reaction for combining fragments of an antibody and functional means within a single molecule allows for obtaining antibody-drug conjugates (ADC conjugates) with a relatively homogenous indicator of distribution of antibody-drug relations (DAR).
11 cl, 10 ex, 9 tbl, 12 dwg
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