FIELD: biotechnology.
SUBSTANCE: proposed is a method for isolating a master seed yeast strain expressing cell wall asparaginase and exhibiting an asparagine-reducing activity in non-inducing conditions, including repeated cycles of adaptive evolution and mutagenesis, followed by strain selection. Also proposed are master seed yeast strains of Saccharomyces cerevisiae produced by said method and expressing cell wall asparaginase, exhibiting an asparagine-reducing activity in non-inducing conditions.
EFFECT: above yeast is used to reduce the content of asparagine in the process of preparation or processing of food products.
31 cl, 18 dwg, 11 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| FUNCTIONAL IMPROVEMENT OF MICROORGANISMS TO MINIMIZE ACRYLAMIDE PRODUCTS | 2011 |
|
RU2603061C2 |
| MODIFIED TSA GENE ENCODING THE THERMOSTABLE L-ASPARAGINASE FROM THERMOCOCCUS SIBIRICUS ARCHAEA, RECOMBINANT ESCHERICHIA COLI STRAIN - THERMOSTABLE L-ASPARAGINASE PRODUCER AND METHOD FOR PURIFICATION OF THIS ENZYME | 2022 |
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| METHOD FOR REDUCING OF ACRYLAMIDE FORMATION IN HEAT TREATED FOODSTUFF, OBTAINED FOODSTUFF AND APPLICATION OF ASPARAGINASE IN SAID METHOD | 2003 |
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