FIELD: biotechnology.
SUBSTANCE: proposed is a method for isolating a master seed yeast strain expressing cell wall asparaginase and exhibiting an asparagine-reducing activity in non-inducing conditions, including repeated cycles of adaptive evolution and mutagenesis, followed by strain selection. Also proposed are master seed yeast strains of Saccharomyces cerevisiae produced by said method and expressing cell wall asparaginase, exhibiting an asparagine-reducing activity in non-inducing conditions.
EFFECT: above yeast is used to reduce the content of asparagine in the process of preparation or processing of food products.
31 cl, 18 dwg, 11 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| FUNCTIONAL IMPROVEMENT OF MICROORGANISMS TO MINIMIZE ACRYLAMIDE PRODUCTS | 2011 |
|
RU2603061C2 |
| METHOD FOR IMPROVING CATALYTIC PROPERTIES OF HYPERTHERMOPHILIC TYPE I L-ASPARAGINASE, TYPE I HYPERTHERMOPHILIC ASPARAGINASE OBTAINED BY SUCH METHOD | 2023 |
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| ASPARAGINASE OBTAINED FROM BASIDIOMYCETES | 2011 |
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| AMIDOHYDROLASES FOR PREPARING FOODSTUFFS OR STIMULANTS | 2008 |
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| RECOMBINANT THERMOSTABLE L-ASPARAGINASE PRODUCER STRAIN FROM MELIORIBACTER ROSEUS | 2022 |
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| ACRYLAMIDE CONTENT DECREASE METHOD | 2009 |
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| FROST-RESISTANT YEAST AND ITS APPLICATION | 2017 |
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RU2756307C2 |
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RU2415605C1 |
