FIELD: biotechnology.
SUBSTANCE: disclosed is a recombinant strain Escherichia coli BL21 (DE3) that is a producer of type II thermostable L-asparaginase from Meliobacter roseus (MrAII), obtained by introducing the synthesized mrAII gene corresponding to the sequence SEQ ID NO: 2, in vector pET-28a(+) under control of T7 promoter into recipient strain Escherichia coli BL21 (DE3).
EFFECT: invention provides obtaining thermostable type II L-asparaginase from Meliobacter roseus (MrAII), exhibiting hydrolysis activity with respect to L-asparagine with specific activity of 1530 U/mg.
1 cl, 3 dwg, 2 tbl, 7 ex
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