FIELD: genetic engineering.
SUBSTANCE: invention relates to the field of genetic engineering, biochemistry and biotechnology, in particular to a modified tsA_mod gene encoding a thermostable L-asparaginase from the archaean Thermococcus sibiricus. The nucleotide sequence of said gene is shown in SEQ ID NO: 1. The present invention also relates to a recombinant strain of Escherichia coli BL21(DE3)/pME2020. real strain is a producer of thermostable L-asparaginase. The strain was obtained by introducing the specified modified tsA_mod gene as part of the pME2020 vector into the E. coli BL21(DE3) recipient strain.
EFFECT: invention makes it possible to efficiently obtain the enzyme L-asparaginase with a high degree of purification; thermostable L-asparaginase can be used in biomedicine and food industry.
2 cl, 2 dwg, 1 tbl
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