FIELD: biotechnology.
SUBSTANCE: present invention relates to the field of biotechnology, in particular to a method for producing a conjugate. The invention discloses an option of conjugation of an antibody and a cytotoxic agent through a bifunctional linker, wherein the cytotoxic agent is preliminarily bound to the linker, after which the reaction mixture is used without purification to bind to the antibody. The method described in the invention has an advantage for the preparation of stably bound conjugates with effector and reporter groups.
EFFECT: this conjugation method provides for high yield of conjugates of high purity and homogeneity without inter-chain cross-linking and inactivated linker residues.
19 cl, 1 tbl, 25 dwg, 8 ex
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