FIELD: biotechnology.
SUBSTANCE: described is a method for multiplex profiling of chromosomes in biological samples using target-specific DNA probes, involving the following steps: a) recovering, extracting or extracting DNA from biological samples; b) connecting a plurality of specific DNA probes with sequences of specific DNA targets on at least one specific chromosome in an intact interphase cell; c) attaching fluorescent labels capable of emitting distinct signals to portions of a plurality of DNA probes such that a final DNA probe generates one to four fluorescent signals, thereby creating a unique signal, characterized in that the unique signal composition is different from the targets on any given chromosome; and d) hybridizing DNA probes with chromosome targets; f) obtaining images of hybridized targets using fluorescent cubes for detecting fluorescent colors.
EFFECT: invention widens range of means for profiling chromosomes.
15 cl, 5 ex, 1 tbl, 13 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD AND DEVICE FOR DETERMINING CHROMOSOME STRUCTURE | 2005 |
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| MULTIPLEX DETECTION OF NUCLEIC ACIDS | 2014 |
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| METHODS, CARRIERS AND KITS FOR IMPROVED ANALYSIS OF COMPARATIVE GENOMIC HYBRIDISATION | 2016 |
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| DETECTION OF CHROMOSOME ANOMALIES IN CELLS OBTAINED FROM FOLLICULAR FLUID | 2005 |
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| NUCLEIC ACID PROBE AND A METHOD OF DETECTING GENOME FRAGMENTS | 2014 |
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| METHOD FOR EVALUATING MUTAGENIC IMPACTS | 2002 |
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| RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS | 2006 |
|
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| RECOMBINANT PLASMID DNA ALPHA-R-12 DESIGNATED FOR MARKING 6-TH HUMAN CHROMOSOME IN METAPHASE IN INTERPHASE CELLS | 2004 |
|
RU2265060C1 |
| METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING | 2011 |
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RU2671980C2 |
| HIGHLY MULTIPLEX PCR METHODS AND COMPOSITIONS | 2012 |
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RU2650790C2 |
