FIELD: biotechnology.
SUBSTANCE: proposed is a method for cultivating aerobic methane-assimilating microorganisms Methylococcus capsulatus RNCIM B-13554 in a flow mode with a constant rate of collection of the culture fluid and a passage rate of the mineral medium from 0.25 to 0.35 h-1 at 41 to 44°C, pH 5.4 to 5.8, and the flow rate of the methane:air gas-air mixture of 1:3, wherein nickel is additionally introduced into the nutrient medium and up to 10% of water is replaced with a centrate of the culture fluid; the concentration of components of mineral nutrition is established depending on the set passage rate of the medium D and the predicted productivity of the cultivation process Y based on the formula.
EFFECT: invention provides an increase in the productivity of the process of synthesis of biomass in bioreactors with different mass transfer characteristics when scaling the process of long-term cultivation.
3 cl, 4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CULTIVATING METHANE-OXIDIZING MICROORGANISMS | 2023 |
|
RU2811437C1 |
| METHOD FOR PRODUCING A BIOMASS OF METHANE-OXIDISING MICROORGANISMS AND A LINE FOR PRODUCTION THEREOF | 2020 |
|
RU2755539C1 |
| METHYLOCOCCUS CAPSULATUS STRAIN IS A PRODUCER OF HIGH-PROTEIN BIOMASS | 2022 |
|
RU2787202C1 |
| METHYLOCOCCUS CAPSULATUS MC19 STRAIN - PRODUCER OF PROTEIN MASS | 2021 |
|
RU2760288C1 |
| METHYLOCOCCUS CAPSULATUS VKPM B-13479 STRAIN - PRODUCER OF MICROBIAL PROTEIN MASS, RESISTANT TO AGGRESSIVE MEDIUM | 2019 |
|
RU2728345C1 |
| METHOD FOR PREPARING BIOMASS | 0 |
|
SU908085A1 |
| METHOD OF GROWING METHANE-OXIDIZING MICROORGANISMS | 1989 |
|
RU2032737C1 |
| METHOD OF PRODUCING CAROTENOID-ENRICHED PROTEIN BIOMASS ON NATURAL GAS USING STRAIN OF METHANE-OXIDISING BACTERIA METHYLOMONAS KOYAMAE B-3802D | 2023 |
|
RU2822163C1 |
| FERMENTATION PLANT FOR CULTIVATION OF METHANE-OXIDIZING BACTERIA METHYLOCOCCUS CAPSULATUS | 2020 |
|
RU2743581C1 |
| FERMENTATION APPARATUS FOR METHANE-ASSIMILATING MICROORGANISMS | 2015 |
|
RU2580646C1 |
