FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, namely to producing yeast strains - producers of recombinant proteins. Proposed is a recombinant plasmid DNA pGAP3tcGFP, represented in SEQ ID NO 1, for expressing a marker recombinant protein GFP. Also proposed is a Yst-pGAP3tcGFP strain of methylotrophic yeast P.pastoris, produced by transforming cells of methylotrophic yeast P.pastoris of the GS115 strain, for producing a marker recombinant protein GFP containing said recombinant plasmid DNA inside the cells. Also proposed is a method for producing a target recombinant protein using the above strain.
EFFECT: high level of production of the target protein.
6 cl, 6 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHYLOTROPHIC YEAST STRAIN PICHIA PASTORIS YST-HSA-PDI1, PRODUCING RECOMBINANT HUMAN SERUM ALBUMIN | 2019 |
|
RU2733423C1 |
| METHYLOTROPHIC YEAST STRAIN PICHIA PASTORIS YST-PPIC9PGAPZALPHA-SHORT_HTFNG, PRODUCING RECOMBINANT HUMAN TRANSFERRIN CONTAINING AMINO ACID SUBSTITUTES N413D AND N611D | 2019 |
|
RU2723084C1 |
| RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I | 2015 |
|
RU2605326C1 |
| DNA MOLECULE FOR EXPRESSION OF BILE SALT-STIMULATED LIPASE | 1996 |
|
RU2157847C2 |
| MICROBIOLOGICAL SYNTHESIS METHOD OF TARGET RELEASED PROTEIN IN YEAST Saccharomyces cerevisiae | 2012 |
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| PREPARATION OF TRITICUM AESTIVUM WHEAT PROTEINASE OBTAINED IN SOLUBLE FORM AND METHOD FOR PREPARATION THEREOF | 2017 |
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| PREPARATION OF RECOMBINANT HUMAN SERUM ALBUMIN AND METHOD OF ITS PRODUCTION | 2006 |
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| METHOD FOR PRODUCING PHOSPHOLIPASE А2 KOMAGATAELLA PHAFFII (PICHIA PASTORIS) YIB ΔLEU2_PLA2S PRODUCTION STRAIN | 2020 |
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RU2746817C1 |
| DNA FRAGMENT CORRESPONDING TO GENE SKC-2, METHOD FOR EXPRESSING DNA FRAGMENT CORRESPONDING TO GENE SKC-2, PLASMID VECTOR PEKG3 PROVIDING FOR EXPRESSING DNA FRAGMENT CORRESPONDING TO GENE SKC-2, PLASMID VECTOR PES KC-4 PROVIDING FOR EXPRESSING DNA FRAGMENT CORRESPONDING TO GENE SKC-2, ESCHERICHIA COLI BACTERIA STRAIN HAVING PLASMID VECTOR PEKG3 USABLE FOR EXPRESSING DNA FRAGMENT CORRESPONDING TO GENE SKC-2, AND STREPTOKINASE PRODUCED IN UTILIZING ESCHERICHIA COLI BACTERIA STRAIN HSK-M | 1991 |
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RU2107726C1 |
| ANTIBODIES BINDING TUMOR-ASSOCIATED MUC1, AND METHODS OF THEIR OBTAINING | 2016 |
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RU2652901C1 |
