METHOD FOR PRODUCING HIGHLY PRODUCTIVE YEAST STRAINS Russian patent published in 2022 - IPC C12N1/19 C12N15/81 

Abstract RU 2770894 C1

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology, namely to producing yeast strains - producers of recombinant proteins. Proposed is a recombinant plasmid DNA pGAP3tcGFP, represented in SEQ ID NO 1, for expressing a marker recombinant protein GFP. Also proposed is a Yst-pGAP3tcGFP strain of methylotrophic yeast P.pastoris, produced by transforming cells of methylotrophic yeast P.pastoris of the GS115 strain, for producing a marker recombinant protein GFP containing said recombinant plasmid DNA inside the cells. Also proposed is a method for producing a target recombinant protein using the above strain.

EFFECT: high level of production of the target protein.

6 cl, 6 dwg, 3 ex

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RU 2 770 894 C1

Authors

Bobik Tatiana Vladimirovna

Gabibov Aleksandr Gabibovich

Kostin Nikita Nikolaevich

Mokrushina Iuliana Anatolevna

Smirnov Ivan Vitalevich

Smirnov Ivan Vitalevich

Dates

2022-04-25Published

2020-12-11Filed