FIELD: biotechnology.
SUBSTANCE: invention relates to the field of molecular biology and biotechnology and is a biologically active protein preparation, having specific activity of papain-like cysteine proteinases, characterized in that it is an amino acid sequence selected from SEQ ID NO:2-4, expressed in soluble form. Invention also relates to a method for producing a biologically active protein preparation, wherein the method comprises transforming cells with plasmids containing DNA, encoding a protein having an amino acid sequence selected from SEQ ID NO: 2-4, cultivation and isolation of a biologically active preparation.
EFFECT: invention provides the possibility of obtaining a Triticum aestivum preparation with a high and stable yield, a high level of purification and functional activity.
9 cl, 8 dwg, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF WHEAT CYSTEINE PROTEASE PROTEINS FAMILY (TRITICUM AESTIVUM) PRODUCING AND PREPARATION OF TRITIKAIE-ALPHA PROTEIN, OBTAINED USING SAID METHOD | 2015 |
|
RU2603054C2 |
| METHOD FOR INCREASING ACTIVITY OF TRITICAINE-ALPHA | 2020 |
|
RU2740319C1 |
| METHOD OF PRODUCING A RECOMBINANT NUCLEASE CAS ESSENTIALLY FREE OF BACTERIAL ENDOTOXINS, THE PREPARATION OBTAINED BY THIS METHOD AND CONTAINING A KIT FOR USE IN A CRISPR/Cas SYSTEM | 2019 |
|
RU2707542C1 |
| RECOMBINANT PLASMID DNA PNDCTR1 CODING HYBRID POLYPEPTIDE GST-NDCTR1, ESCHERICHIA COLI BL21 (DE3)/PNDCTR1 BACTERIA STRAIN - PRODUCER OF HYBRID POLYPEPTIDE GST-NDCTR1 AND HYBRID POLYPEPTIDE GST-NDCTR1, MAKING CHELATES OF COPPER, SILVER AND PLATINUM IONS | 2015 |
|
RU2603092C1 |
| METHOD FOR OBTAINING CASPASE-3 | 2022 |
|
RU2792795C1 |
| PLASMID 40Gal DETERMINING SYNTHESIS OF α-GALACTOSIDASE α-PsGal, STRAIN Ecoli Rosetta(DE3)/40Gal - PRODUCER OF CHIMERIC PROTEIN CONTAINING AMINO-ACID SEQUENCE α-PsGal, AND METHOD FOR ITS PRODUCTION | 2012 |
|
RU2504583C1 |
| PLASMID 40NaGal, DETERMINING SYNTHESIS OF α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, STRAIN E.coli Rosetta(DE3)/40NaGal - PRODUCER OF CHIMERIC PROTEIN, WHICH INCLUDES AMINOACID SEQUENCE OF RECOMBINANT α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, AND METHOD OF ITS OBTAINING | 2013 |
|
RU2525682C1 |
| RECOMBINANT BACTERIAL STRAIN ESCHERICHIA COLI ROSETTA 2(DE3)/pET28с-Mau - PRODUCER OF DNA POLYMERASE A | 2023 |
|
RU2815455C1 |
| THERMOSTABLE ALCOHOLDEHYDROGENASE FROM ARCHAEA THERMOCOCCUS SIBIRICUS | 2009 |
|
RU2413766C1 |
| T7 PHAGE DNA-DEPENDENT RNA-POLYMERASE ENZYME PRODUCER STRAIN | 2022 |
|
RU2825469C2 |
