FIELD: biotechnology.
SUBSTANCE: invention relates to a type II CRISPR/Cas9 genome editing system, characterized in that the system contains the Cas9 protein, auxiliary proteins, crRNA (CRISPR RNA) and tracrRNA (transactivated CRISPR RNA) in the functional form of the RNP complex (ribonucleoprotein complex) Cas9 protein and guide RNA formed by hybridization of crRNA with tracrRNA.
EFFECT: invention is effective for DNA editing.
22 cl, 14 dwg, 11 ex
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