FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to a mouse containing an expression cassette Cas9 genomically integrated into locus Rosa26, wherein the expression cassette Cas9 contains an encoding sequence for protein Cas9, to a cell of the specified mouse, as well as to its production method.
EFFECT: invention is effective for testing a capability of CRISPR/Cas9-nuclease containing protein Cas9 and guiding RNA of modifying a target genomic locus in vivo, as well as for optimization of the capability of CRISPR/Cas9-nuclease of modifying the target genomic locus in vivo.
67 cl, 27 dwg, 12 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CRISPR/CAS PLATFORM FOR EXCLUSIVE SCREENING FOR DETECTING GENETIC VULNERABILITIES ASSOCIATED WITH TAU PROTEIN AGGREGATION | 2020 |
|
RU2808829C2 |
| NON-HUMAN ANIMALS CONTAINING HUMANISED ALBUMIN LOCUS | 2020 |
|
RU2815514C2 |
| MICE CONTAINING MUTATIONS, AS A RESULT OF WHICH FIBRILLIN-1 SHORTENED AT C-TERMINUS IS EXPRESSED | 2017 |
|
RU2721125C1 |
| NEW ENZYMES AND CRISPR SYSTEMS | 2016 |
|
RU2777988C2 |
| CRISPR/CAS SCREENING PLATFORM FOR IDENTIFICATION OF GENETIC MODIFIERS OF SEEDING OR AGGREGATION OF TAU PROTEIN | 2020 |
|
RU2819350C2 |
| SYSTEM DESIGN, METHODS AND OPTIMIZED GUIDE COMPOSITIONS FOR SEQUENCE MANIPULATION | 2013 |
|
RU2796017C2 |
| NEW CRISPR ENZYMES AND SYSTEMS | 2016 |
|
RU2771826C2 |
| COMPONENTS OF THE CRISPR-CAS SYSTEM, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION | 2013 |
|
RU2796549C2 |
| NOVEL ENZYMES AND CRISPR SYSTEMS | 2016 |
|
RU2737537C2 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM DEMEQUINA SEDIMINICOLA BACTERIA | 2019 |
|
RU2722933C1 |
