USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS Russian patent published in 2020 - IPC C12Q1/68 

Abstract RU 2724470 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and describes a new bacterial nuclease of the CRISPR-Cas9 system of P. pneumotropica, as well as its use to form strictly specific double-strand breaks in a DNA molecule. Said nuclease has unusual properties and can be used to change the sequence of genomic DNA in a cell of a unicellular or multicellular organism.

EFFECT: thereby increasing universality of available CRISPR-Cas9 systems, which will make it possible to use different versions of Cas9 nuclease for cutting genome or plasmid DNA in different organisms, in more specific sites and/or in different conditions.

8 cl, 3 tbl, 4 ex, 14 dwg

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RU 2 724 470 C1

Authors

Severinov Konstantin Viktorovich

Shmakov Sergey Anatolievich

Artamonova Daria Nikolaevna

Goryanin Ignat Igorevich

Musharova Olga Sergeevna

Andreeva Julia Valerevna

Zyubko Tatiana Igorevna

Fedorova Iana Vitalevna

Khodorkovskii Mikhail Alekseevich

Pobegalov George Evgenevich

Arseniev Anatoliy Nikolaevich

Selkova Polina Anatolevna

Vasilieva Aleksandra Andreevna

Artamonova Tatiana Olegovna

Abramova Marina Viktorovna

Dates

2020-06-23Published

2019-11-11Filed