FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. A Fab fragment of an antibody against human CEACAM5 has been proposed. A conjugate including the specified Fab fragment, a composition and a method for diagnosing cancer using a conjugate are also proposed, in particular, for the diagnosis of colorectal cancer, breast cancer, lung cancer, thyroid cancer or cancer resulting from metastasis of these malignant neoplasms. In addition, polynucleotides, expression vectors and host cells have been proposed to produce Fab fragments and conjugates according to the invention.
EFFECT: invention provides binding of the Fab fragment to the detectable substance in the absence of weakening of the binding activity against human CEACAM5, as well as accumulation of a conjugate including the Fab fragment in the tumor lesion site for a short time after administration.
36 cl, 4 dwg, 1 tbl, 6 ex
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