FIELD: molecular biology, virology and biotechnology.
SUBSTANCE: invention relates to the field of molecular biology, virology and biotechnology. A set of reagents for the detection of SARS-CoV-2 RNA by real-time loop isothermal amplification using a one-step reverse transcription reaction combined with real-time loop isothermal amplification using developed unique oligonucleotide primers in a freeze-dried state with an analytical sensitivity of 1⋅103 copies of SARS-CoV-2 RNA in 1 ml, without the steps of sample preparation and RNA isolation. The kit contains a freeze-dried analytical reaction mixture packaged in striped microtubes, a control freeze-dried reaction mixture packaged in striped microtubes, a diluent, a positive control sample, a negative control sample, a buffer for extracting a biological sample, a medical disposable sterile probe.
EFFECT: invention can be used for specific clinical laboratory diagnostics of coronavirus infection COVID-19, namely for detection of RNA of severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 in analyzed samples.
1 cl, 3 tbl, 1 ex
| Title | Year | Author | Number |
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| SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2 | 2021 |
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|
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| OLIGONUCLEOTIDES FOR DETECTION OF SARS-CoV-2 MUTATION S:N501Y | 2022 |
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|
RU2731390C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF MUTATION S:delVYY143-145 OF SARS-CoV-2 | 2022 |
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| TEST SYSTEM FOR DETECTING SPECIFIC NUCLEOTIDE SEQUENCES CHARACTERISTIC OF THE DETECTED MICROORGANISMS OR VIRUSES, METHOD FOR APPLICATION OF THE TEST SYSTEM (OPTIONS) | 2021 |
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| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFICATION OF HUMAN CORONAVIRUS 2019-nCoV RNA BY PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2020 |
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RU2734300C1 |
