FIELD: biotechnology.
SUBSTANCE: invention relates to molecular biology, virology and biotechnology, particularly to genetic engineering. Described is a test system for detecting RNA virus SARS-CoV-2, comprising primer SBT0017 5'-GCTGGCACAGACTTAGAAGGT-3', primer SBT0018 5'-AGCAGCGTACAACCAAGCTAA-3', probe SBT0019 5'-GTTGACAGGCAAACAGCACAAGCAGCTG-3', for positive control, GAPDH-specific primers SBT0014 5'-TCCAAAATCAAGTGGGGCGA-3', primer SBT0015 5'-TGATGACCCTTTTGGCTCCC-3', probe SBT0016 5'-CGTCTTCACCACCATGGAGAAGGCTG-3', or ACTB-specific primer SBT0011 5'-CGTCTTCCCCTCCATCGTG-3', primer SBT0012 5'-AGGGTGAGGATGCCTCTCTT-3', probe SBT0013 5'-GATGGTGGGCATGGGTCAGAAGGATTC-3'. Also described is a test system for detecting RNA virus SARS-CoV-2, comprising primer SBT003 5'-GTTCGCATTCAACCAGGACAG-3', primer SBT004 5'-ACCTTCTAAGTCTGTGCCAGC-3' and probe SBT0026 5'- CTGGTGTTTACCAATGTGCTATGAGGCCC-3', for positive control, GAPDH-specific primers SBT0014 5'-TCCAAAATCAAGTGGGGCGA-3', primer SBT0015 5'-TGATGACCCTTTTGGCTCCC-3', probe SBT0016 5'-CGTCTTCACCACCATGGAGAAGGCTG-3', or ACTB-specific primer SBT0011 5'-CGTCTTCCCCTCCATCGTG-3', primer SBT0012 5'-AGGGTGAGGATGCCTCTCTT-3', probe SBT0013 5'-GATGGTGGGCATGGGTCAGAAGGATTC-3'. Disclosed is a method of detecting RNA virus SARS-CoV-2 using said test systems.
EFFECT: invention can be used for detection of genetic material (RNA) of coronavirus SARSCoV-2 associated with respiratory disease COVID-19.
3 cl, 3 dwg, 6 tbl, 5 ex
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