PERSONALIZED VACCINE Russian patent published in 2022 - IPC A61K39/00 A61P35/00 

FIELD: medicine.

SUBSTANCE: group of inventions relates to a personalized anticancer vaccine, its production method, and use. The vaccine contains a recombinant virus of a smallpox vaccine, encoding from 6 to 35 peptides, where each peptide is a fragment of a neoantigen containing a neoepitope, as well as a flanking sequence(s) on one or both sides of this neoepitope, and it contains at least one tumor-specific mutation appeared together with neoplastic conversion and/or cancer cell progression. The production method includes (1) a stage of identification of 6 to 35 peptides suitable for encoding by the recombinant virus of the smallpox vaccine, including: a) extraction of DNA from a tumor sample and a non-tumorous sample, b) selection of target areas, c) their sequencing, d) identification of one or more tumor-specific mutations by means of comparison of DNA sequences obtained from the specified tumor samples and non-tumorous samples; and (2) a stage of production of the recombinant virus of the smallpox vaccine, including the production of synthetic nucleic acid encoding selected peptides in the form of one or more fusion from 2-15 peptides, and the production of a recombinant peptide-expressing virus of the smallpox vaccine.

EFFECT: use of a vaccine allows for the treatment of cancer or prevention of its relapses, providing stimulation of an immune response of T-cells.

38 cl, 12 dwg, 8 tbl, 5 ex