METHOD FOR CONSTRUCTING RECOMBINANT POXVIRUS FOR THERAPEUTIC VACCINE Russian patent published in 2024 - IPC C12N15/863 A61K39/00 

FIELD: biotechnology.

SUBSTANCE: described is a method of constructing a recombinant poxvirus containing one or more expression cassettes, each of which is intended for expression of a peptide or a fusion product of a plurality of peptides, characterized in that it involves carrying out, by means of processing means (11) of server (1), the following steps: (a) selecting a first subset of candidate peptides from a set of candidate peptides, wherein the candidate peptides of the first subset of the candidate peptides exhibit transmembrane scores below the transmembrane score threshold, where the transmembrane score of the candidate peptide indicates the probability that the candidate peptide represents or forms at least one transmembrane segment within its sequence; (b) determining an optimum distribution of candidate peptides of said first subset by expression cassette (s) among a plurality of possible distributions, where said optimal distribution is, if there are at least two expression cassettes, the smallest range between the hydrophobicity scores of the at least two expression cassettes; (c) for each expression cassette, determining the optimum slot assignment of candidate peptides of said first subset as a function of the cassette slot occupancy rule so as to select a peptide fusion product with the lowest transmembrane score, wherein said cassette slot occupancy rule determines possible positions of candidate peptide slots within the expression cassette in accordance with transmembrane scores of candidate peptides and, in case of equal transmembrane scores, in accordance with scores of hydrophobicity of candidate peptides; (d) determining a DNA transfer sequence containing a nucleotide sequence of one or more expression cassettes to obtain said recombinant poxvirus. Described is a method for preparing a therapeutic vaccine for treating infectious diseases or proliferative diseases, involving: implementing a method for constructing a recombinant poxvirus; obtaining said recombinant poxvirus; obtaining a composition containing a therapeutically effective amount of said recombinant poxvirus and a pharmaceutically acceptable carrier.

EFFECT: invention increases output of recombinant poxvirus.

12 cl, 10 dwg, 7 tbl