FIELD: biotechnology.
SUBSTANCE: present invention relates to a set of cartridges for the determination of a status of nucleic acid methylation; to a system for the determination of nucleic acid methylation in a biological sample; to a method for the production of bisulfite-converted DNA; to a method for the detection of cancer or predisposition to cancer in a subject, as well as to a kit for the determination of DNA methylation, including the above-mentioned set of cartridges.
EFFECT: invention allows for carrying out the whole process at one stage: DNA purification, incubation with bisulfite, desulphurization, the second DNA purification, and methyl-specific PCR; it is also possible to carry out the analysis of different types of samples with the assessment of changes in methylation, observed at different types of cancer diseases.
32 cl, 37 dwg, 16 tbl, 16 ex
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