COMPOSITIONS, KITS AND METHODS FOR DETECTING VIRAL SEQUENCES Russian patent published in 2024 - IPC C12Q1/70 

FIELD: biotechnology.

SUBSTANCE: described is a composition for amplifying three different target sequences in the genome of SARS-CoV-2, containing: a first forward primer and a first reverse primer configured to amplify a first target sequence present in a first target region of the SARS-CoV-2 genome, wherein the first target sequence contains at least 10 contiguous nucleotides of the first target region; a second forward primer and a second reverse primer configured to amplify a second target sequence present in a second target region of the genome of SARS-CoV-2, wherein the second target sequence contains at least 10 contiguous nucleotides of the second target region; and a third forward primer and a third reverse primer configured to amplify a third target sequence present in a third target region of the SARS-CoV-2 genome, wherein the third target sequence contains at least 10 contiguous nucleotides of the third target region; wherein each of the first, second and third target areas has less than 50% similarity with a similar area in bat-SL-CoVZC45.

EFFECT: invention enables the specific and differential detection of SARS-CoV-2 viruses in samples, including veterinary samples, clinical samples, food samples, forensic samples, environmental samples (for example, obtained from soil, garbage, waste water, air, water, surfaces of food and industrial industries, and so forth) or a biological sample obtained from a human or non-human animal.

15 cl, 5 dwg, 37 tbl, 10 ex