METHOD FOR PRODUCTION OF ACTIVE FORM OF LONG-ACTING INSULIN ANALOGUE DERIVATIVE, USING CLOSTRIPAIN Russian patent published in 2022 - IPC C07K14/62 C12P21/02 C07K1/14 A61K38/28 

Abstract RU 2781307 C2

FIELD: biotechnology.

SUBSTANCE: present invention relates to the field of biotechnology, specifically to the production of insulin derivatives; it can be used in medicine for the treatment of diabetes. The proposed invention allows for the production of an active form of a long-acting insulin analogue derivative in such a way that the insulin analogue can be converted into the active form without cleavage of B-chain, even when it interacts with clostripain. In a traditional method for conversion of proinsulin into an active form by means of using trypsin, an albumin-binding domain is cleaved, hindering the conversion of the long-acting insulin analogue derivative into the active form.

EFFECT: production method, according to the present invention, overcomes the specified obstacle, and, thereby, it can be effectively used for the production of a long-acting therapeutic means for the treatment of diabetes.

14 cl, 12 dwg, 5 tbl, 10 ex

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RU 2 781 307 C2

Authors

Akhn, Ken Khoon

Dzhon, Sonkeon

Eon, Chkhekha

Dates

2022-10-11Published

2019-07-19Filed