ANIMAL MODEL OF ZINC-DEPENDENT AMYLOIDOGENESIS IN ALZHEIMER'S DISEASE Russian patent published in 2022 - IPC G09B23/28 

FIELD: medical technology.

SUBSTANCE: invention relates to pathological physiology and concerns the creation of an animal model of zinc-dependent amyloidogenesis in the pathogenesis of Alzheimer’s disease. To do this, C. elegans transgenic nematodes of the CL2120 line (dvis 14 [(pCL12) unc-54::beta 1-42 +(pCL26) mtl-2::GFP]) are grown, overexpressing human 42-membered beta-amyloid (Aβ42), to the L4 stage at 15°C on NGM plates with bacterial lawn E. coli OP50. Next, nematodes are placed in a drop of 0.2 ml of M9 buffer containing ZnSO₄ 20 mcm and isoD7-A42 40 mcm, and cultured for 4 hours at 25°C with stirring. Then nematode drops are transferred to Petri dishes with NMGZn, with bacterial lawn E. coli OP50 and cultured in a Petri dish at 25°C.

EFFECT: invention provides the creation of an effective animal model of zinc-dependent amyloidogenesis in the pathogenesis of Alzheimer’s disease.

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