FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to a method for modifying a target locus of interest, including the delivery to the specified locus of a composition, not found in nature or constructed, containing effector CRISPR-Cas protein of type VI, class 2, containing 2 HEPN domains, and one or more components, which are nucleic acids. A composition, not found in nature or constructed, for modifying RNA of interest, as well as a delivery system containing it for modifying the target locus of interest, and a vector system for modifying the target locus of interest, including one or more vectors, are also disclosed. The invention also relates to a cell for modifying RNA of interest, as well as to a cell line containing it.
EFFECT: invention is effective for modification of a target locus of interest.
60 cl, 146 dwg, 12 tbl, 9 ex
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