FIELD: biochemistry.
SUBSTANCE: invention relates to a constructed, non-naturally occurring system based on short palindromic repeats, regularly located in groups (CRISPR)-CRISPR-associated enzyme (Cas) (CRISPR-Cas), for modification of target locus of interest. Also disclosed are methods of modifying, a method of producing a modified plant, a method of identification using said system. A host cell containing said system is also disclosed.
EFFECT: invention enables to produce a modified plant efficiently.
52 cl, 116 dwg, 10 tbl, 19 ex
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