FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, specifically to the production of recombinant polypeptides of botulinum neurotoxin (hereinafter – BoNT); it can be used in medicine for the treatment of a condition related to undesired neuronal activity. BoNT polypeptides with a modified receptor-binding domain of Clostridium botulinum serotype B, strain 4 (B4-HC), having amino acid mutations, which modify binding of BoNT to human synaptotagmin II (hereinafter – Syt II), are proposed.
EFFECT: invention provides obtainment of BoNT polypeptides with enhanced binding to human Syt II.
44 cl, 24 dwg, 8 tbl, 6 ex
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