METHOD FOR DIAGNOSTICS OF LUNG CANCER Russian patent published in 2022 - IPC G01N33/497 G16H50/20 A61B5/08 

FIELD: medicine; oncology.

SUBSTANCE: invention relates to medicine, namely to oncology; it can be used for diagnostics of lung cancer. Levels of biomarkers in a sample of exhaled air are measured by gas chromatography with mass-spectrometry detection (hereinafter – GC-MS) with preliminary concentration of volatile organic compounds in sorption tubes with Tenax TA sorbent, using GC-MS system equipped with a two-stage thermal desorber. At the first stage of thermal desorption, volatile organic compounds are extracted from the tube with the concentrated sample, for 5 min, at a temperature of 195°C. At the second stage of thermal desorption, the resulting gas mixture is focused in a trap with its subsequent heating from -10 to 195°C. Separation of volatile organic compounds is carried out on a capillary column with an immobile polystyrene-divinyl benzene phase. As biomarkers, ratios of peak areas of volatile organic compounds are used, namely: hexane : 2-pentanone, toluene : acetone, 1-pentanol : acetone, pentanal : 2-pentanone, dimethyl trisulfide : dimethyl disulfide, nonanal : 2,3-butanedione, heptane : allyl methyl sulfide, 2-butaneone : 2-pentanone, isoprene : acetone, 1-methylthioproane : acetone, dimethyl sulfide : acetone, acetonitrile : acetone, having the greatest correlation coefficients. They are used for creation of a classification model, using a technology of artificial neural networks.

EFFECT: method provides a possibility of reduction in time of analysis, increase in the reliability of the method due to reduction in time of thermal desorption, reduction in a temperature, and use of values of ratios of peak areas of volatile organic compounds as parameters.

1 cl, 1 dwg, 4 tbl, 3 ex