FIELD: biology, in particular gene engineering, microbiological industry, production of new beta-lactam antibiotics.
SUBSTANCE: constructed is recombinant plasmid DNA pETTvDAO2 providing high level of DNA fragment which encodes D-amino acid oxydase of Trigonopsis variabilis yeast in Escherichia coli cells. As a result of transformation of E.coli strain with disclosed recombinant plasmid and selection of transformed clones new recombinant Escherichia coli strain C41(DE3)/pETTvDAO2 as producer D-amino acid oxydase is obtained.
EFFECT: improved method for producing of cephalosporin antibiotics.
2 cl, 4 dwg, 2 tbl, 7 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd |
2006 |
- Redo Vadim Aleksandrovich
- Ehl'Darov Mikhail Anatol'Evich
- Khatuntseva Svetlana Anatol'Evna
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310688C1 |
| RECOMBINANT PLASMIDE pNhyDAAO PROVIDING SYNTHESIS OF D-AMINO ACID OXIDASE OF NATRONOSPORANGIUM HYDROLYTICUM ACPA39 (NhyDAAO) BACTERIA IN ESCHERICHIA COLI CELLS AND RECOMBINANT STRAIN ESCHERICHIA COLI BL21(DE3)/pNhyDAAO VKPM V-143 37 - NhyDAAO PRODUCER |
2022 |
- Tishkov Vladimir Ivanovich
- Atroshenko Denis Leonidovich
- Golovina Diana Igorevna
- Elcheninov Aleksandr Gennadevich
- Kublanov Ilya Valerevich
- Pometun Anastasiya Aleksandrovna
- Savin Svyatoslav Sergeevich
|
RU2803295C1 |
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2005 |
- Khatuntseva Svetlana Anatol'Evna
- Ehl'Darov Mikhail Anatol'Evich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2300566C2 |
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2006 |
- Khatuntseva Svetlana Anatol'Evna
- Ehl'Darov Mikhail Anatol'Evich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2388826C2 |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME |
2015 |
- Eldarov Mikhail Anatolevich
- Sklyarenko Anna Vladimirovna
- Dumina Mariya Vladimirovna
- Satarova Dzhenni Ernstovna
- Zhgun Aleksandr Aleksandrovich
- Medvedeva Natalya Viktorovna
- Yarotskij Sergej Viktorovich
|
RU2624022C1 |
| RECOMBINANT PLASMID PROVIDING SYNTHESIS OF BARNASE IN CELLS OF ESCHERICHIA COLI, ESCHERICHIA COLI STRAIN - PRODUCER OF BARNASE AND METHOD FOR PRODUCING BARNASE |
2017 |
- Deev Sergej Mikhajlovich
- Shulga Aleksej Anatolevich
- Lukyanova Tamara Ivanovna
- Konovalova Elena Valerevna
|
RU2650871C1 |
| RECOMBINANT DNA, CODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND RECOMBINANT PLASMID pAS017, PROVIDING G-CSF SYNTHESIS IN CELLS OF Escherichia coli |
2011 |
- Shul'Ga Aleksej Anatol'Evich
|
RU2529363C2 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
- Shingarova Ljudmila Nikolaevna
- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Jakimov Sergej Aleksandrovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2527171C1 |
| ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN |
2012 |
- Ramazanov Jurij Akhmetovich
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|
RU2512527C2 |
| RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR |
2013 |
- Shingarova Ljudmila Nikolaevna
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2531547C1 |
