FIELD: biotechnology, genetic engineering, biochemistry, antibiotics.
SUBSTANCE: invention proposes a constructed recombinant plasmid pSVH0106 comprising the natural gene sequence for acylase of glutaryl-7-aminocephalosporinic acid of the strain Brevundimonas diminuta BKM B-1297 that encodes a full-scale enzyme precursor. As result of transformation of E. coli strain with the proposed recombinant plasmid and selection of transformed clones the novel strain of E. coli BL21(DE3)/pSVH0106 is obtained that represents a producer of G17ACA acylase providing high yield of active enzyme. Owing to the effective and stable expression of recombinant G17ACA acylase in the proposed system using the proposed invention allows scaling a process for preparing this enzyme used broadly in manufacturing antibiotics.
EFFECT: valuable properties of plasmid.
2 cl, 4 dwg, 2 tbl, 9 ex
| Title |
Year |
Author |
Number |
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